Monday, 25 August 2014

Linker Helps Detect Protein Partners Biochemistry: Cleavable photo-cross-linker aids protein-protein interaction analysis

In this technique, a selenium-based linker is engineered into a bait protein. Photoactivating the linker locks bait and prey proteins together. Oxidatively cleaving it releases the bait, allowing scientists to tag the prey protein for analysis.
Credit: Adapted From J. Am. Chem. Soc.
 Protein-protein interactions play important roles in normal body processes and in disease. Scientists often lock interacting proteins together with cross-linkers to better understand these interactions—information that can help lead to drugs that block or promote the protein-protein interplay. Now, a research team in China has developed a cleavable photo-cross-linking agent that can be genetically engineered into proteins to help study the interactions in living cells.
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